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Antigen retrieval was performed in 10 mM citrate buffer, pH 6.0 using pressure cooker (at 125 C for 5 min)

It provides data on the peptide's absorption, distribution, metabolism, and excretion, which are essential for its potential future clinical development

For comparison, FDA-approved anti-obesity medications like semaglutide 2.4 mg (Wegovy) have cardiovascular outcomes data from the SELECT trial (N=17,604, median follow-up 39.8 months), which demonstrated a 20% reduction in major adverse cardiovascular events [6]

It remains an experimental compound without formal clinical guidelines for dosage or usage

Robust human mechanism data are not yet available