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Cells were washed once in ice-cold PBS and lysed into RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM EDTA, 1% sodium deoxycholate, 1% Triton X-100, 0.1% sodium dodecyl sulfate [SDS]) with the addition of 1X Halt Protease and Phosphatase Inhibitor Cocktail (PI, ThermoFisher Scientific, #78440)

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237 In an open clinical trial, 20 subjects with high perceived stress levels and mild-to-moderate anxiety and sleep disturbance were treated with two 300 mg doses of lemon balm extract per day for 15 days