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Therefore, we speculated that CBS is nonfunctional under supraphysiological cystine concentrations and investigated its function in a customized culture media containing cystine at a physiological concentration of 50 M or less
Briefly, SCD Day 10 erythroblasts were cultured at indicated conditions, and Cystine-FITC was added to cultured SCD erythroblasts at 100 M for 45 min, allowing uptake
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In the first reaction, glutamatecysteine ligase (GCL
