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Briefly, LX-2 cells were grown to a confluence of 60-70% on glass coverslips, washed three times with PBS, fixed in 4% formaldehyde for 20 min, permeabilized in 0.5% Triton X-100 for 5 min and blocked with Duolink blocking solution for 60 min at 37 C

When human cells are integrated into microfluidic platforms that mimic key physiological parameters, including oxygen gradients, fluid flow, and multicellular tissue interactions, these systems provide improved translational relevance than conventional models [175, 176]

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