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PubMed Central, He, Wei, et al

Diabetes mellitus is one of the major risk factor for CAD and other risk factors for CAD are hypertension, obesity, smoking and dyslipoproteinemias [2]

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Immunoblotting Protein samples in 1x NuPage LDS sample buffer, 25 mM DTT (or 50 mM TCEP pH 7.0) were loaded on to SDS PAGE gels (Invitrogen, XP04205) and transferred to nitrocellulose membranes