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Lancet 383:549557 Wang S, Pang T, Gao M, Kang H, Ding W, Sun X, Zhao Y, Zhu W, Tang X, Yao Y, Hu X (2013a) HPV E6 induces eIF4E transcription to promote the proliferation and migration of cervical cancer

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After the addition of 100 L of internal standard (d 4 -nicotine in acetonitrile), 400 L ammonia (5 %) and 10 mL acetonitrile, the mixture was shaken thoroughly (15 min) and ultra-sonified (15 min)