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4.3
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Description

It's extra juice

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Complementary DNA (cDNA) was made by reverse transcription of RNA (2 g) using Moloney murine leukemia virus reverse transcriptase (Promega, Madison, MI, USA) and labeled with Biotin-16-2-deoxy-uridine-5-triphosphate (Roche Diagnostics, Indianapolis, IN, USA)

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Consequently, the aforementioned data suggest that particular pathways may have both local and systemic relevance

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Beta-actin mRNA, which served as an internal control, was co-amplified with 5-HT-1A receptor mRNA to ensure accuracy

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RA: Writing original draft

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