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2.4 Transporter inhibition study using HEK293 cells The procedures for cell culture, dosing solution preparation, cell lysate preparation, and sampling were consistent with those in the substrate study described above, except for the pre-incubation and incubation steps

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In Danio rerio (zebrafish), ibuprofen increased oxidative stress markers, including catalase, glutathione S-transferase, and protein carbonyls ( Dreissena polymorpha (zebra mussel), ibuprofen elicited lipid peroxidation and disrupted enzymatic responses ( Reduced growth in P